Preparation of Leishman stain for Staining Blood Film

Preparation of Leishman stain for Staining Blood Film



1) Leishman stain powder . . . . . . . . . . . . . . . . . 0.6 g

2) Methanol (methyl alcohol) . . . . . . . . . . . 400 ml (The methanol must be water-free.)


1) Weigh the Leishman powder and transfer it to a completely dry brown bottle.

2) Add a few dry glass beads (to assist in dissolving the dye).

3) Using a dry cylinder, measure the methanol and add this to the stain.

4) Mix well at intervals until the dye is completely dissolved.

==> Warming the solution in a 37 C water bath will help the dye to dissolve.

5) Label the bottle and mark it Flammable and Toxic.

6) Store it at room temperature in the dark.

For use: Filter 50–100 ml of the stain into a stain dispensing container which can be closed when not in use


When kept tightly stoppered, the stain is stable for several weeks.

Moisture must not be allowed to enter the stain.

Caution: Methanol is toxic and highly flammable, therefore handle it with care well away from an open flame.


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