Techniques for blood film staining for Differential White Blood Cell Count – Manually


أقرأ الترجمة بالبلدى

The Index

Introduction ——————————————————————————- Page 1

1) Value of the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Page 1

2) The principle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Page 1

Preparation of Thin Blood Film ——————————————————— Page 2

1) Materials and Reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Page 2

2) Specimen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Page 2

3) Method for Preparations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Page 3

4) Drying of the film . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Page 4

5) Writing the Name and number of the patient: . . . . . . . . . . . . . . . . . . . . . . . . Page 4

6) Fixation of the film . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  Page 4

Staining of The Blood Film ————————————————————— Page 5

Method One: Leishman staining technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . Page 5

Method Two: Writ’s stain technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Page 6

Method Three: May-Grunwald and Giemsa stains technique . . . . . . . . . . . .Page 7

Method Four: MField stain Technique ….. (Rapid) . . . . . . . . . . . . . . . . . . . . . Page 8

How to remedy poor results ————————————————————— Page 9

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Method Four: MField stain Technique ….. (Rapid)  
Reagents
The needed materials and tools in
page 2 but we will talk about the reagents only in this section

Method:

Click here for Field Stain A and B preparation

1) Fix the thin film with methanol for 2–3 minutes.

2) Dip the slide into Field stain B and count up to five.


3) Drain and wash the slide in the first container of tap water .

4) Drain and dip the slide into Field stain A and count up to 10.

5) Drain and wash the slide well in the second container of tap water.

4) Examine the color of the film. It should appear mauve, neither too blue nor too pink.

  • If the film is not satisfactory, return the slide either to the Field stain A or to the Field stain B for a few more seconds, as needed.
  • If the film is satisfactory, stand the slide in a draining rack to dry.

Dip the slide into Field stain B   wash with tap water   Drain and dip the slide into Field stain A and count up to 10   wash with tap water2   examine the blood film

How to remedy poor staining results ===> Page 9

طريقة معالجة الفلم لو اتصبغ غلط ===> Page 9

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