Preparation of Wright’s stain for blood film staining

Preparation of Wright’s stain for blood film staining

wright's stain

Materials:

  • Wright stain powder. . . . . . . . . . . . . . . .  . . . . . . . . . . . . . . . . . . . 1.0 gm
  • Methanol (methyl alcohol) (must be water-free) . . . . . . . . . . . 400 ml

Method:

1) Weigh the Wright powder and transfer it to a dry brown bottle.

2) Add a few glass beads (to assist in dissolving the dye).

3) Using a dry cylinder, measure the methanol and add this to the stain.

4) Mix well at intervals until the powder is completely dissolved.

5) Warming the solution in a 37 C water bath will help the dye to dissolve.

6) Label the bottle and mark it Flammable and Toxic.

7) Store it at room temperature in the dark. When kept tightly stoppered, the stain is stable for several weeks. Moisture must not be allowed to enter the stain.

 

For use:

  • Allow 3–5 days before using freshly made stain to allow time for the stain to ‘ripen’.
  • Filter 50–100 ml of the stain into a stain dispensing container which can be closed when not in use.

Caution: Methanol is toxic and highly flammable, therefore handle it with care well away from an open flame.

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