Preparation of White Blood Cell diluting fluid for WBC count

 Preparation of White Blood Cell diluting fluid

Component:

–          Acetic acid, glacial . . . . . . . . . . . . . . . . . . . . . . 2 ml

–          Distilled water. . . . . . . . . . . . . . . . . . . . . . . . . 98 ml

–          Gentian violet, 1% w/v . . . . . . . . . . . . . . . . . . 2 ml


Procedure:

To make 100 ml solution:

1) Prepare the Gentian Violet solution by dissolving 0.1 g gentian violet in 10 ml distilled water and filter.

2) Fill a 100 ml cylinder to the 98 ml mark with distilled water.

3) Add 2 ml concentrated (glacial) acetic acid and mix.

4)       Add the gentian violet solution and mix.

Caution:

Glacial acetic acid is a corrosive chemical with an irritating vapor  therefore handle it with care and in a well ventilated room.

Do not mouth-pipette (use a pipette filler or use a variable micropipette).

Storage:

Transfer to a storage bottle and label.

Store in the dark at room temperature.

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