Preparation of May–Grünwald Stain for Blood Film Staining

Preparation of May–Grünwald Stain for Blood Film Staining

Material:

  • Powdered May–Grünwald powder stain . . . . . . . . . . . . . . . . . . . . . 5 gm
  • Methanol (CH3OH) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . q.s. 1000 ml (q.s. means “bring volume up to”)

Procedure:

1) Weigh the Giemsa powder and transfer it to a completely dry brown bottle.

2) Add a few dry glass beads (to assist in dissolving the dye).


3) Using a dry cylinder, measure the methanol and add this to the stain.

4) Mix well at intervals until the dye is completely dissolved (4 consecutive days).

==> Warming the solution in a 37 C water bath will help the dye to dissolve.

5) Label the bottle and mark it Flammable and Toxic.

6) Store it at room temperature in the dark.

For use: Filter 50–100 ml of the stain into a stain dispensing container which can be closed when not in use

Notes:

  1. When kept tightly stoppered, the stain is stable for several weeks.
  2. Moisture must not be allowed to enter the stain.

Caution: Methanol is toxic and highly flammable, therefore handle it with care well away from an ope

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