Preparation of Leishman stain for Staining Blood Film


Preparation of Leishman stain for Staining Blood Film

leishman-s-stain-500x500

Materials:

1) Leishman stain powder . . . . . . . . . . . . . . . . . 0.6 g

2) Methanol (methyl alcohol) . . . . . . . . . . . 400 ml (The methanol must be water-free.)

Procedure:

1) Weigh the Leishman powder and transfer it to a completely dry brown bottle.

2) Add a few dry glass beads (to assist in dissolving the dye).


3) Using a dry cylinder, measure the methanol and add this to the stain.

4) Mix well at intervals until the dye is completely dissolved.

==> Warming the solution in a 37 C water bath will help the dye to dissolve.

5) Label the bottle and mark it Flammable and Toxic.

6) Store it at room temperature in the dark.

For use: Filter 50–100 ml of the stain into a stain dispensing container which can be closed when not in use

Notes:

When kept tightly stoppered, the stain is stable for several weeks.

Moisture must not be allowed to enter the stain.

Caution: Methanol is toxic and highly flammable, therefore handle it with care well away from an open flame.

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