Preparation of Giemsa Stain for Blood Film Staining

Preparation of Giemsa Stain for Blood Film Staining

Giemsa_stain

Material:

  • Powdered Giemsa stain . . . . . . . . . . . . . . . . . . . . . . . . 3.8 gm
  • Methanol (methyl alcohol) (CH3OH) . . . . . . . . . . . . . . . . 250ml
  • Glycerol (methyl alcohol)(C3H8O3) . . . . . . . .   . . . . . . . . 250ml

Procedure:

1) Weigh the Giemsa powder and transfer to a 500ml dry brown bottle.

2) Add a few dry glass beads (to assist in dissolving the dye).

==>Note: If water enters to the stock solution during its preparation or storage, the stain will be spoilt

3) Measure the methanol using a clean dry cylinder, and add to the stain. Mix well.

4) Measure the glycerol using a clean dry cylinder, and add to the stain. Mix well.

5) Mix well at intervals until the dye is completely dissolved (4 consecutive days).

==> Warming the solution in 50 – 60oC water bath or 37oC water bath will help the dye to dissolve.

6) Label the bottle “GIEMSA STAIN”and mark it as Flammable and Toxic.

Storage and stability:

  • Store at room temperature in the dark.
  • The stain is stable for several months, if kept well-stoppered.

Caution: Methanol is toxic and highly flammable, therefore, handle it with care well away from an open flame



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