Preparation of Fouchet’s reagent for Bilirubin test in urine

Preparation of Fouchet’s reagent for Bilirubin test in urine

Reagents:

To make 100 ml:

Trichloroacetic acid (TCA) ……………………… 25 g
Iron III chloride (ferric chloride) 100 g/l (10% w/v), ………….. 10 ml (dissolve 10 g of the chemical in 100 ml of distilled water)
Distilled water …………………………………. to 100 ml


Method

1) Rapidly weigh the TCA in a beaker. Add 30–40 ml of distilled water, and stir to dissolve the chemical.

Caution: TCA is a strongly corrosive and deliquescent chemical, therefore avoid contact with the eyes and skin and make sure the
stock bottle of chemical is tightly stoppered after use.

2) Using a funnel, transfer the dissolved acid to a 100 ml volumetric flask or if unavailable, a measuring cylinder.

3) Add the iron III chloride solution, and mix. Make up to the 100 ml mark with distilled water, and mix well.

4) Transfer the reagent to a small brown bottle (one in which a dropper can be inserted).

5) Label the bottle, and mark it Corrosive. Store at room temperature. The reagent is stable for several months.

Comments

Facebook Comments

Leave a Reply