Preparation of Filed stain for Blood film staining

Preparation of Filed stain for Blood film staining

field stain

Preparation of Field stain A

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1) Preparation from prepared powders

Materials:

  • Field stain A powder . . . . . . . . . . . . . . . .5 gm
  • Distilled water, heated to 80 °C . . . . . . . . q.s. 600ml

Method:

1) Mix until dissolved.

2) Filter when cool into a 1000-ml bottle.

3) Label the bottle “FIELD STAIN A” and write the date.

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2) Preparation from original stains and chemicals

Materials:

  • Methylene blue (medicinal) . . . . . . . . . . . . . . . . . . . . . . . . . 1.6 gm
  • Azur 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 gm
  • Disodium hydrogen phosphate (Na2HPO4), anhydrous . . . . . . . . 10.0 gm
  • Potassium dihydrogen phosphate (KH2PO4), anhydrous . . . . . . . 12.5 gm
  • Distilled water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . q.s. 1000ml

Method:

1) Dissolve the two salts in the distilled water.

2) Pour about half of the solution into a 1000-ml bottle containing a few glass beads.

3) Add the stain powders and mix well. 

4) Add the remainder of the solution.

5) Mix well and filter into a clean 1000-ml bottle.

6) Label the bottle “FIELD STAIN A” and write the date.

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Preparation of Field stain B

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1) Preparation from prepared powders

Materials:

  • Field stain B powder . . . . . . . . . . . . . . . . . . . . . . . . . 4.8 gm
  • Distilled water, heated to 80 °C . . . . . . . . . . . . . . . . . q.s. 600ml

 

Method:

1) Mix until dissolved.

2) Filter when cool into a 1000-ml bottle.

3) Label the bottle “FIELD STAIN B” and write the date.

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2) Preparation from original stain and chemicals

Materials:

  • Eosin (yellow water-soluble) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.0 gm
  • Disodium hydrogen phosphate (Na2HPO4), anhydrous . . . . . . . . . . . . . . . 10.0 gm
  • Potassium dihydrogen phosphate (KH2PO4), anhydrous . . . . . . . . . . . . . . 12.5 gm
  • Distilled water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .q.s. 1000ml

Method:

1) Dissolve the two salts in the distilled water.

2) Pour into a 1000-ml bottle.

3) Add the eosin. Mix until dissolved.

4) Filter into a clean 1000-ml bottle.

5) Label the bottle “FIELD STAIN B” and write the date.

Undiluted, Field stains can be used for as long as they give good results. After dilution, they should be filtered every 2–3 days.

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