Preparation of Erhlich’s reagent for Urobilinogen detection in urine

Preparation of Erhlich’s reagent for Urobilinogen detection in urine

Reagent

To make about 200 ml:

4-Dimethylaminobenzaldehyde …………………. 4 g
Hydrochloric acid, concentrated ……………. 40 ml
Distilled water ……………………………………… 160 ml


Method

1 Weigh the 4-dimethylaminobenzaldehyde, and transfer it to a leak-proof brown bottle.

2 Measure the water and add to the chemical. Mix to dissolve.

3 Add the concentrated hydrochloric acid, and mix well.
Caution: Concentrated hydrochloric acid is a corrosive chemical with an injurious vapour, therefore handle it with great care in a well ventilated room.

4) Label the bottle and mark it Corrosive. Store at room temperature. The reagent is stable for several weeks if protected from daylight.

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