Preparation of Drapkin’s solution in Your Lab

Drapikin’s solution preparation in the lab:

==> Drabkin diluting fluid can be prepared from commercially available reagent tablets.

==> The instructions for its preparation are supplied by the manufacturer.

Procedure:

For laboratories equipped with an accurate balance, Drabkin diluting fluid can be prepared as follows:

1) Prepare the following weights from each solid:

  • Potassium ferricyanide (hexacyanoferrate 111) . . 200 mg

  • Potassium cyanide . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 mg

  • Potassium dihydrogen phosphate . . . . . . . . . . . . . . 140 mg (buffer to adjust Ph to 7.2)

  • Non-ionic detergent (e.g. Nonidet, Triton-X-100, Sterox SE) . . . 1 ml

  • Distilled or deionized water. . . . . . . . . . . . . . . . . . to 1 litre

2) Dissolve the first three chemicals in the distilled water and mix.

3) Add the detergent and mix gently.

==> The reagent should be clear and pale yellow in colour.

==> When measured against water as blank in a spectrophotometer at a wavelength of 540nm, the absorbance should be zero.

4) Transfer the diluting fluid to a brown bottle.

5) Label the bottle “DRABKIN DILUTING FLUID” and write the date.

==> If the reagent appears cloudy, discard.

Precautions:

==>  Potassium cyanide is a highly poisonous chemical and should be used only by experienced chemists.

==> When not in use it should be kept in a locked cupboard.

==> After using the chemical, wash your hands thoroughly.



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