Preparation of CPD and ACD-NIHS

Preparation of CPD and ACD-NIH A

1) Preparation of CPD (Citrate phosphate dextrose):

Chemicals needed:

==> Trisodium citrate dehydrate 26.3g

==> Citric acid monohydrate 3.27g

==> Sodium dihydrogen phosphate monohydrate 2.22 g

==> Dextrose 25.5 g

Procedure:

1) dissolve the solids in 1 liter distilled water.

2) The solution is sterilized by autoclaving at 126˚C for 30 minutes. Its PH is 5.6-5.8.

3) for use, 7 volumes of blood are added to 1 volume of solution. (e.g. 7 ml blood to 1 ml soluiton)



2) Preparation of ACD (NIH A):

ACD (NIH-A): The following formula was recommended by the United States National Institute of Health; hence referred to a “NIH-A” solution.

Chemicals needed:

==> Trisodium citrate dihydrate 22 g

==> Citric acid monohydrate 8 g

==> Dextrose 25 g

Procedure:

1) Dissolve the solids in 1 liter of distilled water.

2) The solution is sterilized by autoclaving at 126˚C for 30 minutes. Its pH is 5.4.

3) For use, 10 volumes of blood are added to 1.5 volumes of solution.

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