laboratory reagents: Sodium Nitroprusside dry reagent for Ketone test in urine

Preparation of Sodium Nitroprusside dry reagent for Ketone test in urine

Method To make about 153 g:


Reagents:-

Ammonium sulphate ………………………. 100 g
Sodium carbonate, anhydrous ………….. 50 g
Sodium nitroprusside* …………………… 3 g
The accepted international name for sodium nitroprusside is sodium nitrosopentacyanoferrate III.

Procedure:

1) Weigh the sodium nitroprusside, and transfer it to a wide-necked, plastic, screw-cap container.
Caution: Sodium nitroprusside is a poisonous chemical, therefore handle it with care.

2) Using a pestle or the end of a clean test tube (made of thick glass), grind the crystals to a powder.

3) Weigh the sodium carbonate and ammonium sulphate, and transfer these to the container.

4) Cap the container and mix the powders by shaking.

5) Label the container and mark it Toxic. Store at room temperature. The reagent is stable for several months. Mix the reagent before use.

Comments

Facebook Comments

Leave a Reply