Alanine aminotransferase (ALT or sGPT) test|Colorimetric method|Spinreact kit

Alanine aminotransferase (ALT or GPT) test

Liver Function Panel

Colorimetric Technique

By spinreact kits

I) Introduction

1) Names:-

Formal name: Alanine Aminotransferase and abbreviated as (ALT)

Also known as: Serum Glutamic-Pyruvic Transaminase; SGPT; GPT; Alanine Transaminase.

2) What is the ALT?

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3) Why it is required?

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4) When should you get tested?

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5) What are the needed preparations before the test?

Read about it in topic of ALT test definition and Patient education.CLICK HERE

II) Required sample:-

1) Sample type:-

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2) Sample collection method:-

Read about it in topic of ALT test by colorimetric technique – the general conceptCLICK HERE

3) Criteria for sample rejection:-

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III) The Test:-

Colorimetric technique

By spinreact kits

1) Principle

Read about it in topic of ALT test by colorimetric technique – the general conceptCLICK HERE

2) Devices used:-

Read about it in topic of ALT test by colorimetric technique – the general conceptCLICK HERE

3) Reagents:-



Kits of ALT in spinreact company with reference id 1001175.

  • Composition:-
    • Reagent 1 (R1) (substrate): L-alanine 200 mmol/L, α-ketoglutarate 2 mmol/L, pH 7.4.
    • Reagent 2 (R2) (coloring reagent): 2,4-dinitrophenylhydrazine (DNPH)1 mmol/L.
    • Primary calibrator of Pyruvic acid 2 mmol/L.
    • NaOH (0.4N)… this to make alkaline medium required for the reaction of the pyruvate with the 2,4-DNPH.
  • STORAGE
    • Store at 2-8ºC.
    • Reagents are stable until the expiry date shown on the label when stored tightly closed, protected from light and contaminations prevented during their use.
  • Indications of deterioration:
    • Presence of particles and turbidity.
  • Reagent preparation
    • All reagents are ready to use.


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